1NT2
CRYSTAL STRUCTURE OF FIBRILLARIN/NOP5P COMPLEX
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-C |
| Synchrotron site | APS |
| Beamline | 14-BM-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 42 21 2 |
| Unit cell lengths | 125.308, 125.308, 87.482 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 * - 2.900 |
| R-factor | 0.257 |
| Rwork | 0.257 |
| R-free | 0.31600 * |
| Structure solution method | MAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.390 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.970 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.122 * | 0.400 * |
| Total number of observations | 75425 * | |
| Number of reflections | 12451 | |
| <I/σ(I)> | 11.7 | 1.3 |
| Completeness [%] | 78.5 | 20.5 * |
| Redundancy | 4.7 | 1.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8.6 | 295 | PEG, pH 8.6, VAPOR DIFFUSION, temperature 295K |
| 1 | Vapor diffusion, hanging drop * | 8.6 | 295 | PEG, pH 8.6, VAPOR DIFFUSION, temperature 295K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | Tris-HCl | 100 (mM) | pH8.6 |
| 2 | 1 | reservoir | trisodium citrate | 200 (mM) | |
| 3 | 1 | reservoir | PEG400 | 35-40 (%(w/v)) | |
| 4 | 1 | drop | protein | 20-30 (mg/mol) |
