1NT2

CRYSTAL STRUCTURE OF FIBRILLARIN/NOP5P COMPLEX

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-BM-C
Synchrotron site	APS
Beamline	14-BM-C
Temperature [K]	100
Detector technology	CCD
Detector	ADSC QUANTUM 4
Wavelength(s)	1.0
Spacegroup name	P 42 21 2
Unit cell lengths	125.308, 125.308, 87.482
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 * - 2.900
R-factor	0.257
Rwork	0.257
R-free	0.31600 *
Structure solution method	MAD
RMSD bond length	0.008
RMSD bond angle	1.390 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.970
High resolution limit [Å]	2.900	2.900
Rmerge	0.122 *	0.400 *
Total number of observations	75425 *
Number of reflections	12451
<I/σ(I)>	11.7	1.3
Completeness [%]	78.5	20.5 *
Redundancy	4.7	1.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	8.6	295	PEG, pH 8.6, VAPOR DIFFUSION, temperature 295K
1	Vapor diffusion, hanging drop *	8.6	295	PEG, pH 8.6, VAPOR DIFFUSION, temperature 295K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	Tris-HCl	100 (mM)	pH8.6
2	1	reservoir	trisodium citrate	200 (mM)
3	1	reservoir	PEG400	35-40 (%(w/v))
4	1	drop	protein	20-30 (mg/mol)