1NT0

Crystal structure of the CUB1-EGF-CUB2 region of MASP2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	CCD
Detector	ADSC QUANTUM 315
Wavelength(s)	0.979
Spacegroup name	P 1 21 1
Unit cell lengths	70.411, 103.901, 70.484
Unit cell angles	90.00, 119.93, 90.00

Refinement procedure

Resolution	40.000 * - 2.700
Rwork	0.248
R-free	0.28200 *
Structure solution method	SIRAS
RMSD bond length	0.007
RMSD bond angle	26.500 *
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SOLVE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000 *	2.770
High resolution limit [Å]	2.700	2.700
Rmerge	0.086 *	0.346 *
Number of reflections	23873
Completeness [%]	98.7	99.6
Redundancy	2.8 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.2 *	298	Protein: 2-5 mg/ml, 50 mM Tris pH=8.2, 10 mM CaCl2 and 0.5 M NaCl. Reservoir: 0.8-1.0 M Na2-Tartrate, 80 mM Li2SO4 and 0.1 MES pH=6.5, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	2-5 (mg/ml)
2	1	drop	Tris	50 (mM)	pH8.2
3	1	drop		10 (mM)
4	1	drop		0.5 (M)
5	1	reservoir	sodium tartrate	0.8-1.0 (M)
6	1	reservoir		80 (mM)
7	1	reservoir	MES	0.1 (M)	pH6.5