1NSU

Crystal structure of galactose mutarotase from Lactococcus lactis mutant H96N complexed with galactose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	277
Detector technology	AREA DETECTOR
Collection date	2002-08-19
Detector	SIEMENS HI-STAR
Wavelength(s)	1.54178
Spacegroup name	P 21 21 21
Unit cell lengths	44.900, 76.200, 210.800
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 1.800
R-factor	0.176
Rwork	0.173
R-free	0.23600
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	1l7j
RMSD bond length	0.013
RMSD bond angle	2.320
Data reduction software	SAINT
Data scaling software	SAINT
Phasing software	TNT
Refinement software	TNT

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.880
High resolution limit [Å]	1.800	1.800
Rmerge	0.065 *
Number of reflections	64786
<I/σ(I)>	11.9	3.2
Completeness [%]	95.0	95
Redundancy	2.5	1.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	6	4 *	MES, methylether PEG-5000, NaCl, pH 6.00, VAPOR DIFFUSION, HANGING DROP, temperature 277K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG5000 MME	15-19 (%)
2	1	reservoir	MES	100 (mM)	pH6.0
3	1	drop	enzyme	17.5 (mg/ml)