1NSQ
MECHANISM OF PHOSPHATE TRANSFER BY NUCLEOSIDE DIPHOSPHATE KINASE: X-RAY STRUCTURES OF A PHOSPHO-HISTIDINE INTERMEDIATE OF THE ENZYMES FROM DROSOPHILA AND DICTYOSTELIUM
Experimental procedure
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 115.750, 115.750, 98.520 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 8.000 - 2.180 |
| R-factor | 0.18 |
| Rwork | 0.180 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.590 |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 15.100 |
| High resolution limit [Å] | 2.170 |
| Rmerge | 0.077 * |
| Total number of observations | 184822 * |
| Number of reflections | 32198 |
| Completeness [%] | 79.0 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8 * | 18 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | PEG6000 | 5 (%) | |
| 2 | 1 | drop | 20 (mM) | ||
| 3 | 1 | drop | Tris-HCl | 50 (mM) | |
| 4 | 1 | reservoir | PEG6000 | 10 (%) |
