1NSG

THE STRUCTURE OF THE IMMUNOPHILIN-IMMUNOSUPPRESSANT FKBP12-RAPAMYCIN COMPLEX INTERACTING WITH HUMAN FRAP

Experimental procedure

Detector technology	AREA DETECTOR
Collection date	1997-03-01
Detector	XUONG-HAMLIN MULTIWIRE
Spacegroup name	P 21 21 21
Unit cell lengths	46.300, 52.400, 102.000
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	? - 2.200
R-factor	0.196
Rwork	0.196
R-free	0.26500
RMSD bond length	0.006
RMSD bond angle	25.500 *
Data reduction software	UCSD
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
High resolution limit [Å]	2.200 *	2.200 *
Rmerge	0.087	0.270 *
Total number of observations	30805 *
Number of reflections	12865
Completeness [%]	97.4	90.8 *
Redundancy	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	8 *		used to seeding *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	Tris-HCl	20 (mM)
2	1	drop		150 (mM)
3	1	reservoir	PEG8000	15-20 (%)
4	1	reservoir	MPD	5-10 (%)
5	1	reservoir	Tris-HCl	100 (mM)