1NRV
Crystal structure of the SH2 domain of Grb10
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2001-05-09 |
| Detector | CUSTOM-MADE |
| Wavelength(s) | 0.9790 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 28.820, 49.040, 79.610 |
| Unit cell angles | 90.00, 96.62, 90.00 |
Refinement procedure
| Resolution | 30.900 * - 1.650 |
| R-factor | 0.224 |
| Rwork | 0.224 |
| R-free | 0.24000 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1a81 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 23.700 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.690 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.045 | 0.370 |
| Total number of observations | 74325 * | |
| Number of reflections | 26168 | |
| <I/σ(I)> | 10.5 | |
| Completeness [%] | 99.6 | 99.2 |
| Redundancy | 2.84 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 4 * | 12% PEG 8000, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 4 (mg/ml) | |
| 2 | 1 | drop | HEPES | 20 (mM) | pH7.5 |
| 3 | 1 | drop | 100 (mM) | ||
| 4 | 1 | reservoir | PEG8000 | 10 (%) | |
| 5 | 1 | reservoir | 100 (mM) | pH7.5 | |
| 6 | 1 | reservoir | TCEP | 5 (mM) |
