1NRK

YGFZ PROTEIN

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-BM
Synchrotron site	APS
Beamline	17-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2002-04-12
Detector	MARRESEARCH
Wavelength(s)	1.000
Spacegroup name	P 31 2 1
Unit cell lengths	151.000, 151.000, 68.000
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	10.000 - 2.800
R-factor	0.19592
Rwork	0.193
R-free	0.25200 *
Structure solution method	MAD
RMSD bond length	0.013
RMSD bond angle	1.600 *
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	MLPHARE
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.900
High resolution limit [Å]	2.800	2.800
Rmerge	0.055	0.440 *
Number of reflections	21998	2412 *
<I/σ(I)>	22.4	2.1
Completeness [%]	98.5	97.1
Redundancy	4.9	4.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5	295	0.1M Na Acetate, 30% Ammonium Sulfate, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	15 (mg/ml)
2	1	reservoir	ammonium sulfate	30 (%sat)
3	1	reservoir	sodium acetate	0.1 (M)	pH4.5