1NRJ
Signal Recognition Particle Receptor Beta-Subunit in Complex with the SRX Domain from the Alpha-Subunit
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X9A |
Synchrotron site | NSLS |
Beamline | X9A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-07-12 |
Detector | MARRESEARCH |
Wavelength(s) | 1.300 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 78.200, 123.700, 48.800 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 25.000 * - 1.700 |
R-factor | 0.19603 |
Rwork | 0.194 |
R-free | 0.22900 * |
Structure solution method | SAD |
RMSD bond length | 0.026 |
RMSD bond angle | 1.950 * |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SnB |
Refinement software | REFMAC (5.1.19) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 * | |
High resolution limit [Å] | 1.700 * | 1.700 * |
Rmerge | 0.044 * | 0.350 * |
Total number of observations | 444199 * | |
Number of reflections | 52263 * | |
Completeness [%] | 98.7 * | 97.4 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, sitting drop * | 7.5 * | 25 * | 40% (w/v) PEG 3350, 15% (v/v) ethylene glycol, 0.1M ammonium chloride, 0.1M sodium acetate, pH 5.5, Microbatch under oil, temperature 298K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 20 (mg/ml) | |
2 | 1 | reservoir | HEPES | 10 (mM) | pH7.5 |
3 | 1 | reservoir | 150 (mM) | ||
4 | 1 | reservoir | dithiothreitol | 5 (mM) | |
5 | 1 | reservoir | 1 (mM) |