1NRJ

Signal Recognition Particle Receptor Beta-Subunit in Complex with the SRX Domain from the Alpha-Subunit

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X9A
Synchrotron site	NSLS
Beamline	X9A
Temperature [K]	100
Detector technology	CCD
Collection date	2002-07-12
Detector	MARRESEARCH
Wavelength(s)	1.300
Spacegroup name	P 21 21 2
Unit cell lengths	78.200, 123.700, 48.800
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.000 * - 1.700
R-factor	0.19603
Rwork	0.194
R-free	0.22900 *
Structure solution method	SAD
RMSD bond length	0.026
RMSD bond angle	1.950 *
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SnB
Refinement software	REFMAC (5.1.19)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000 *
High resolution limit [Å]	1.700 *	1.700 *
Rmerge	0.044 *	0.350 *
Total number of observations	444199 *
Number of reflections	52263 *
Completeness [%]	98.7 *	97.4 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	7.5 *	25 *	40% (w/v) PEG 3350, 15% (v/v) ethylene glycol, 0.1M ammonium chloride, 0.1M sodium acetate, pH 5.5, Microbatch under oil, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	reservoir	HEPES	10 (mM)	pH7.5
3	1	reservoir		150 (mM)
4	1	reservoir	dithiothreitol	5 (mM)
5	1	reservoir		1 (mM)