1NR7
Crystal structure of apo bovine glutamate dehydrogenase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-01-01 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 96.460, 172.060, 440.750 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 * - 3.300 |
| R-factor | 0.2181 |
| Rwork | 0.218 |
| R-free | 0.29200 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 * |
| RMSD bond angle | 1.260 * |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.300 |
| High resolution limit [Å] | 3.300 | 3.200 |
| Rmerge | 0.044 * | 0.110 * |
| Number of reflections | 102240 | |
| Completeness [%] | 74.5 * | 43.1 * |
| Redundancy | 2.4 * | 1.6 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.8 | 298 | sodium phosphate, sodium chloride, PEG 8000, MPD, sodium azide, OBG, pH 6.8, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | glycerol suspension | 50 (%) | |
| 2 | 1 | drop | sodium phosphate | 0.1 (M) | pH6.7-6.8 |
| 3 | 1 | drop | protein | 10 (mg/ml) | |
| 4 | 1 | reservoir | PEG8000 | 10 (%(w/v)) | |
| 5 | 1 | reservoir | 0.8 (M) | ||
| 6 | 1 | reservoir | MPD | 4 (%(v/v)) | |
| 7 | 1 | reservoir | sodium azide | 1 (mM) | |
| 8 | 1 | reservoir | sodium phophate | 0.1 (M) | pH6.7-6.8 |
| 9 | 1 | reservoir | octyl-beta-glucopyranoside | 0.75-1.5 (%(w/v)) |
