1NQT
Crystal structure of bovine Glutamate dehydrogenase-ADP complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2000-01-01 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 96.460, 171.930, 439.680 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 * - 3.500 |
R-factor | 0.2071 |
Rwork | 0.207 |
R-free | 0.27600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | bovine glutamate dehydrogenase |
RMSD bond length | 0.016 |
RMSD bond angle | 1.920 * |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 * | 3.660 |
High resolution limit [Å] | 3.500 | 3.500 |
Rmerge | 0.040 * | 0.111 * |
Number of reflections | 87616 | |
<I/σ(I)> | 13.7 | 3.5 |
Completeness [%] | 70.7 * | 49.6 * |
Redundancy | 2.3 * | 1.6 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, sitting drop * | 6.8 | 298 | sodium phosphate, PEG 8000 sodium azide, sodium chloride, MPD and OBG, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | glycerol suspension | 50 (%) | |
10 | 1 | reservoir | ADP | 0.3 (mM) | |
2 | 1 | drop | sodium phosphate | 0.1 (M) | pH6.7-6.8 |
3 | 1 | drop | protein | 10 (mg/ml) | |
4 | 1 | reservoir | sodium phosphate | 0.1 (M) | |
5 | 1 | reservoir | octyl-beta-glucopyranoside | 1 (%(w/v)) | |
6 | 1 | reservoir | MPD | 6 (%(v/v)) | |
7 | 1 | reservoir | sodium phosphate | 1 (M) | |
8 | 1 | reservoir | PEG8000 | 14 (%(w/v)) | |
9 | 1 | reservoir | sodium azide | 1 (mM) |