1NQS
Structural Characterisation of the Holliday Junction formed by the sequence d(TCGGTACCGA) at 1.97 A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-05-15 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.811 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 65.588, 23.628, 37.223 |
| Unit cell angles | 90.00, 111.15, 90.00 |
Refinement procedure
| Resolution | 34.710 - 1.970 |
| R-factor | 0.22538 |
| Rwork | 0.223 |
| R-free | 0.27779 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.982 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.710 | 2.080 |
| High resolution limit [Å] | 1.970 | 1.970 |
| Rmerge | 0.070 | 0.296 |
| Number of reflections | 3877 | |
| <I/σ(I)> | 6.1 | 6.1 |
| Completeness [%] | 99.3 | 100 |
| Redundancy | 7.6 | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 290 | MPD, calcium chloride, cacodylate, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 290K |
