1NQP
Crystal structure of Human hemoglobin E at 1.73 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-11-18 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 60.888, 95.814, 99.084 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 14.990 - 1.730 |
| R-factor | 0.191 |
| Rwork | 0.191 |
| R-free | 0.21100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1bbb |
| RMSD bond length | 0.007 |
| RMSD bond angle | 17.800 * |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 1.790 |
| High resolution limit [Å] | 1.730 | 1.730 |
| Rmerge | 0.053 | 0.414 |
| Total number of observations | 390226 * | |
| Number of reflections | 59665 | |
| <I/σ(I)> | 18.6 | |
| Completeness [%] | 97.5 | 89 |
| Redundancy | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 * | 4 * | PEG and Glycerol, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG8000 | 30 (%(w/v)) | |
| 2 | 1 | reservoir | sodium cacodylate | 0.1 (M) | pH6.5 |
| 3 | 1 | reservoir | magnesium acetate | 0.2 (M) | |
| 4 | 1 | reservoir | glycerol | 15-20 (%) | |
| 5 | 1 | drop | protein | 10 (mg/ml) | |
| 6 | 1 | drop | Tris-HCl | 10 (mM) | pH8.0 |
