1NQO

Glyceraldehyde-3-Phosphate Dehydrogenase Mutant With Cys 149 Replaced By Ser Complexed With Nad+ and D-Glyceraldehyde-3-Phosphate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	ENRAF-NONIUS FR591
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1999-03-17
Detector	MAC Science DIP-2030B
Wavelength(s)	1.5418
Spacegroup name	C 1 2 1
Unit cell lengths	140.306, 87.838, 119.410
Unit cell angles	90.00, 119.05, 90.00

Refinement procedure

Resolution	8.000 - 2.010
R-factor	0.194
Rwork	0.193
R-free	0.23500 *
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	1nq5
RMSD bond length	0.006 *
RMSD bond angle	1.400 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.070
High resolution limit [Å]	2.010	2.010
Rmerge	0.105	0.320
Number of reflections	77562 *
<I/σ(I)>	12.1	3.2
Completeness [%]	92.8	74.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.9 *	293	PEG 4000, sodium acetate, Tris-HCl, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	Tris-HCl		pH7.5
2	1	reservoir	PEG4000	10 (%(w/v))
3	1	drop	D,L-G3P	1 (mM)
4	1	drop	NAD	1 (mM)
5	1	drop	TEA	40 (mM)
6	1	drop	EDTA	2 (mM)
7	1	drop	potassium phosphate	50 (mM)	pH8.9