1NQN
Structure of Avm-W110K (W110K mutant of avidin)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-1 |
Synchrotron site | ESRF |
Beamline | ID14-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-06-20 |
Detector | MARRESEARCH |
Wavelength(s) | 0.93 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 67.703, 77.450, 42.894 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.800 |
R-factor | 0.206 |
Rwork | 0.205 |
R-free | 0.22700 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.013 * |
RMSD bond angle | 1.700 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 * | |
High resolution limit [Å] | 1.800 * | 1.800 * |
Rmerge | 0.056 * | 0.261 * |
Number of reflections | 21282 * | |
Completeness [%] | 98.2 * | 98 * |
Redundancy | 2.5 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 * | 20 * | 32% MPEG 2k, 0.1M Tris-HCl, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 20K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 4 (mg/ml) | |
2 | 1 | reservoir | sodium formate | 2.5 (M) | |
3 | 1 | reservoir | acetate | 0.1 (M) | pH4.6 |