1NQN

Structure of Avm-W110K (W110K mutant of avidin)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-1
Synchrotron site	ESRF
Beamline	ID14-1
Temperature [K]	100
Detector technology	CCD
Collection date	2001-06-20
Detector	MARRESEARCH
Wavelength(s)	0.93
Spacegroup name	P 21 21 2
Unit cell lengths	67.703, 77.450, 42.894
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 1.800
R-factor	0.206
Rwork	0.205
R-free	0.22700
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.013 *
RMSD bond angle	1.700 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000 *
High resolution limit [Å]	1.800 *	1.800 *
Rmerge	0.056 *	0.261 *
Number of reflections	21282 *
Completeness [%]	98.2 *	98 *
Redundancy	2.5 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6 *	20 *	32% MPEG 2k, 0.1M Tris-HCl, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 20K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	4 (mg/ml)
2	1	reservoir	sodium formate	2.5 (M)
3	1	reservoir	acetate	0.1 (M)	pH4.6