1NQM

Structure of Savm-W120K, streptavidin mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-1
Synchrotron site	ESRF
Beamline	ID14-1
Temperature [K]	100
Detector technology	CCD
Collection date	1999-01-01
Detector	MARRESEARCH
Wavelength(s)	0.93
Spacegroup name	P 1 21 1
Unit cell lengths	50.433, 100.407, 52.511
Unit cell angles	90.00, 112.12, 90.00

Refinement procedure

Resolution	40.000 - 1.700
R-factor	0.214
Rwork	0.212
R-free	0.24000
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010 *
RMSD bond angle	1.600 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000 *
High resolution limit [Å]	1.700 *	1.700 *
Rmerge	0.041 *	0.290 *
Number of reflections	51529 *
Completeness [%]	97.3 *	98.6 *
Redundancy	2. *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.45	20 *	25% PEG 6K, 0.3M sodium acetate, 0.1M cac. buffer, pH 6.45, VAPOR DIFFUSION, HANGING DROP, temperature 20K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	reservoir	PEG8000	15 (%)
3	1	reservoir	sodium acetate	0.2 (M)
4	1	reservoir	sodium cacodylate	0.1 (M)	pH6.45