1NQJ
CRYSTAL STRUCTURE OF CLOSTRIDIUM HISTOLYTICUM COLG COLLAGENASE COLLAGEN-BINDING DOMAIN 3B AT 1.0 ANGSTROM RESOLUTION IN ABSENCE OF CALCIUM
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-06-01 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 40.637, 57.708, 46.320 |
| Unit cell angles | 90.00, 95.69, 90.00 |
Refinement procedure
| Resolution | 28.900 * - 1.000 |
| R-factor | 0.1416 |
| Rwork | 0.140 |
| R-free | 0.16600 * |
| Structure solution method | MAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 0.035 |
| Data reduction software | SAINT |
| Data scaling software | SAINT |
| Phasing software | CNS |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.000 | 1.070 |
| High resolution limit [Å] | 1.000 * | 1.000 |
| Rmerge | 0.074 * | |
| Number of reflections | 105185 | |
| <I/σ(I)> | 30.7 | 2.94 |
| Completeness [%] | 91.7 | 72.64 |
| Redundancy | 3.01 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 4 * | PEG 3350, LITHIUM CHLORIDE, SODIUM ACETATE, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG3350 | 22-25 (%) | |
| 2 | 1 | reservoir | sodium acetate | 100 (mM) | pH4.6 |
| 3 | 1 | reservoir | 600 (mM) | ||
| 4 | 1 | reservoir | glycerol | 10 (%) | |
| 5 | 1 | drop | protein | 25000 (ng/ml) | |
| 6 | 1 | drop | EGTA | 0.2 (mM) |
