1NQB

TRIVALENT ANTIBODY FRAGMENT

Experimental procedure

Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX9.6
Synchrotron site	SRS
Beamline	PX9.6
Temperature [K]	95
Detector technology	IMAGE PLATE
Collection date	1994-11
Detector	MARRESEARCH
Spacegroup name	H 3
Unit cell lengths	136.320, 136.320, 74.800
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	6.000 - 2.000
R-factor	0.187
Rwork	0.187
R-free	0.23000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1JEL
RMSD bond length	0.008
RMSD bond angle	26.900 *
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.050
High resolution limit [Å]	2.000	2.000
Rmerge	0.053	0.174
Total number of observations	95579 *
Number of reflections	33839
<I/σ(I)>	35.1	12
Completeness [%]	96.6	98.1
Redundancy	2.8	2.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	6.5	4 *	30% 2-PROPANOL, 0.2M NA CITRATE, 0.1M NA CACODYLATE PH 6.5

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	2-propanol	30 (%)
2	1	reservoir	Na cacodylate	0.1 (M)
3	1	reservoir	Na citrate	0.2 (M)