1NQ6
Crystal Structure of the catalytic domain of xylanase A from Streptomyces halstedii JM8
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7B |
| Temperature [K] | 120 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-07-22 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.885 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 34.050, 79.600, 87.800 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.000 * - 1.780 |
| R-factor | 0.14745 |
| Rwork | 0.145 |
| R-free | 0.18000 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1e0w |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.090 |
| Data reduction software | DENZO |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 * | 1.880 |
| High resolution limit [Å] | 1.780 * | 1.780 |
| Rmerge | 0.044 | 0.084 |
| Total number of observations | 78251 * | |
| Number of reflections | 22905 | |
| <I/σ(I)> | 13.1 | 8.9 |
| Completeness [%] | 97.1 | 85.8 |
| Redundancy | 3.4 * | 2.7 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 298 | PEG 4000, MAGNESIUM CHLORIDE, SODIUM ACETATE, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 15 (mg/ml) | |
| 2 | 1 | reservoir | PEG4000 | 10 (%) | |
| 3 | 1 | reservoir | 0.1 (M) | ||
| 4 | 1 | reservoir | sodium acetate | 0.1 (M) | pH5.5 |
