1NQ5

Glyceraldehyde-3-Phosphate Dehydrogenase Mutant With Cys 149 Replaced By Ser Complexed With Nad+

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	ENRAF-NONIUS FR591
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1997-10-15
Detector	MAC Science DIP-2030B
Wavelength(s)	1.5418
Spacegroup name	C 1 2 1
Unit cell lengths	140.480, 87.909, 119.925
Unit cell angles	90.00, 119.00, 90.00

Refinement procedure

Resolution	8.000 - 2.110
R-factor	0.203
Rwork	0.198
R-free	0.24900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1gd1
RMSD bond length	0.005 *
RMSD bond angle	1.400 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	8.000	2.180
High resolution limit [Å]	2.110	2.110
Rmerge	0.085	0.280
Number of reflections	71736 *
<I/σ(I)>	14	3.4
Completeness [%]	99.2	75.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.9 *	293	PEG 4000, sodium acetate, Tris-HCl, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	Tris-HCl		pH7.5
2	1	reservoir	PEG4000	10 (%(w/v))
3	1	drop	D,L-G3P	1 (mM)
4	1	drop	NAD	1 (mM)
5	1	drop	TEA	40 (mM)
6	1	drop	EDTA	2 (mM)
7	1	drop	potassium phosphate	50 (mM)	pH8.9