1NPY
Structure of shikimate 5-dehydrogenase-like protein HI0607
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-07-02 |
| Detector | SBC-2 |
| Wavelength(s) | 0.97921, 0.97935 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 90.578, 66.575, 96.916 |
| Unit cell angles | 90.00, 109.56, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.750 |
| R-factor | 0.1813 |
| Rwork | 0.179 |
| R-free | 0.21900 * |
| Structure solution method | MAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.700 * |
| Data reduction software | d*TREK |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.810 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.059 | 0.360 |
| Number of reflections | 108148 | |
| <I/σ(I)> | 18 | 1.9 |
| Completeness [%] | 98.5 | 88 |
| Redundancy | 4 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 298 | NaCl, NaAcetate, PEG400, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 298 | NaCl, NaAcetate, PEG400, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20 (mg/ml) | |
| 2 | 1 | reservoir | sodium acetate | 0.1 (M) | pH4.6 |
| 3 | 1 | reservoir | 2.0 (M) | ||
| 4 | 1 | reservoir | PEG400 | 10 (%(v/v)) |
