1NPT
Glyceraldehyde-3-Phosphate Dehydrogenase Mutant With Cys 149 replaced by Ala complexed with NAD+
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS FR571 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-06-07 |
| Detector | MAC Science DIP-2030B |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 115.700, 115.700, 224.800 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 8.000 - 2.180 |
| R-factor | 0.18 |
| Rwork | 0.177 |
| R-free | 0.21000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gd1 |
| RMSD bond length | 0.005 * |
| RMSD bond angle | 1.400 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 10.000 | 2.260 |
| High resolution limit [Å] | 2.180 | 2.180 |
| Rmerge | 0.061 | 0.160 |
| Number of reflections | 85949 * | |
| <I/σ(I)> | 16.1 | 5.2 |
| Completeness [%] | 96.1 * | 91 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.9 * | 293 | PEG 4000, sodium acetate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG4000 | 8 (%(w/v)) | |
| 10 | 1 | drop | potassium phosphate | 50 (mM) | pH8.9 |
| 2 | 1 | reservoir | sodium acetate | 0.1 (M) | pH4.6 |
| 3 | 1 | reservoir | EDTA | 2 (mM) | |
| 4 | 1 | reservoir | dithiothreitol | 2 (mM) | |
| 5 | 1 | reservoir | NAD | 1.5 (mM) | |
| 6 | 1 | drop | D,L-G3P | 1 (mM) | |
| 7 | 1 | drop | NAD | 1 (mM) | |
| 8 | 1 | drop | TEA | 40 (mM) | |
| 9 | 1 | drop | EDTA | 2 (mM) |
