1NPS

CRYSTAL STRUCTURE OF N-TERMINAL DOMAIN OF PROTEIN S

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	287
Detector technology	AREA DETECTOR
Collection date	1998-07
Detector	MARRESEARCH
Spacegroup name	P 1 21 1
Unit cell lengths	28.360, 37.940, 37.260
Unit cell angles	90.00, 105.93, 90.00

Refinement procedure

Resolution	20.000 - 1.800
R-factor	0.198 *
Rwork	0.200
R-free	0.23400
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.900
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000
High resolution limit [Å]	1.800
Rmerge	0.080
Number of reflections	7166
Completeness [%]	99.0
Redundancy	2.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.5 *		pH 8.0

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	1.4 (mg/ml)
2	1	reservoir	HEPES	100 (mM)	pH7.5
3	1	reservoir	PEG10000	20 (%(w/v))