1NPO
BOVINE NEUROPHYSIN II COMPLEX WITH OXYTOCIN
Experimental procedure
Source type | ROTATING ANODE |
Source details | RIGAKU RUH3R |
Temperature [K] | 295 |
Detector technology | AREA DETECTOR |
Collection date | 1990-06-06 |
Detector | SIEMENS-NICOLET X100 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 69.050, 69.050, 113.260 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 8.000 - 3.000 |
R-factor | 0.182 |
Rwork | 0.182 |
R-free | 0.26600 |
RMSD bond length | 0.010 |
RMSD bond angle | 2.790 * |
Data reduction software | XENGEN (V. 2.0) |
Phasing software | X-PLOR (3.1) |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 3.180 | |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.056 | 0.253 |
Total number of observations | 38324 * | |
Number of reflections | 5924 | |
<I/σ(I)> | 10.6 | 1.7 |
Completeness [%] | 100.0 | 100 |
Redundancy | 6.4 | 5.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Batch method * | 6 * | 18 * | pH 6.8 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | 1 | NP | 12.5 (mg/ml) | |
2 | 1 | 1 | sodium azide | 0.2 (%(w/v)) | |
3 | 1 | 1 | OT acetate salt | 1.7mg | |
4 | 1 | 1 | ammonium sulfate | saturated, 0.02ml per well |