1NPH

Gelsolin Domains 4-6 in Active, Actin Free Conformation Identifies Sites of Regulatory Calcium Ions

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX14.1
Synchrotron site	SRS
Beamline	PX14.1
Temperature [K]	100
Detector technology	CCD
Collection date	2002-01-31
Detector	ADSC QUANTUM 4
Wavelength(s)	1.488
Spacegroup name	P 41 21 2
Unit cell lengths	122.520, 122.520, 82.723
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.000 - 3.000
Rwork	0.247
R-free	0.29800 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1DB0
RMSD bond length	0.010
RMSD bond angle	1.330
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	AMoRE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	87.700	3.160
High resolution limit [Å]	3.000	3.000
Rmerge	0.120 *	0.310 *
Number of reflections	13141 *
<I/σ(I)>	5.2	2.3
Completeness [%]	99.4 *
Redundancy	6.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	20 *	PEG 8000, calcium chloride, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	Tris-HCl	25 (mM)	pH8.0
2	1	reservoir	PEG8000	15 (%(w/v))
3	1	reservoir		0.1 (mM)
4	1	drop	protein	10 (mg/ml)