1NPE
Crystal structure of Nidogen/Laminin Complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-08-01 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.95370 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 72.180, 75.830, 104.580 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.300 |
| Rwork | 0.223 |
| R-free | 0.25700 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1IJQ and 1KLO |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.500 * |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 * | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.105 | 0.424 |
| Total number of observations | 466114 * | |
| Number of reflections | 25940 * | |
| <I/σ(I)> | 16.7 | 3.4 |
| Completeness [%] | 99.0 | 92.5 |
| Redundancy | 18 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 1 M NaAc, 0.05 M CdSO4, 0.1 M, HEPES pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 13 (mg/ml) | |
| 2 | 1 | reservoir | sodium acetate | 1 (M) | |
| 3 | 1 | reservoir | 0.05 (M) | ||
| 4 | 1 | reservoir | HEPES | 0.1 (M) | pH7.5 |
