1NP2
Crystal structure of thermostable beta-glycosidase from thermophilic eubacterium Thermus nonproteolyticus HG102
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-6B |
Synchrotron site | Photon Factory |
Beamline | BL-6B |
Temperature [K] | 100 |
Detector technology | DIFFRACTOMETER |
Collection date | 2001-12-03 |
Detector | WEISSENBERG |
Wavelength(s) | 1.00 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 66.700, 94.755, 176.720 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 * - 2.400 |
R-factor | 0.23 |
Rwork | 0.230 |
R-free | 0.27200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB code 1BGA |
RMSD bond length | 0.007 |
RMSD bond angle | 1.380 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 * | 2.490 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.182 | 0.327 * |
Number of reflections | 41963 * | |
<I/σ(I)> | 9.9 | 2.2 |
Completeness [%] | 94.0 | 73.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | 291 | He, X.Y., (2001) Acta Crystallogr.,Sect.D, 57, 1650. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 4 (mg/ml) | |
2 | 1 | drop | HEPES | 5 (mM) | pH7.5 |
3 | 1 | reservoir | MPD | 20 (%) | |
4 | 1 | reservoir | 0.2 (M) | ||
5 | 1 | reservoir | sodium acetate | 0.1 (M) | pH4.6 |