1NP2
Crystal structure of thermostable beta-glycosidase from thermophilic eubacterium Thermus nonproteolyticus HG102
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6B |
| Synchrotron site | Photon Factory |
| Beamline | BL-6B |
| Temperature [K] | 100 |
| Detector technology | DIFFRACTOMETER |
| Collection date | 2001-12-03 |
| Detector | WEISSENBERG |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 66.700, 94.755, 176.720 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 * - 2.400 |
| R-factor | 0.23 |
| Rwork | 0.230 |
| R-free | 0.27200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB code 1BGA |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.380 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 * | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.182 | 0.327 * |
| Number of reflections | 41963 * | |
| <I/σ(I)> | 9.9 | 2.2 |
| Completeness [%] | 94.0 | 73.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | 291 | He, X.Y., (2001) Acta Crystallogr.,Sect.D, 57, 1650. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 4 (mg/ml) | |
| 2 | 1 | drop | HEPES | 5 (mM) | pH7.5 |
| 3 | 1 | reservoir | MPD | 20 (%) | |
| 4 | 1 | reservoir | 0.2 (M) | ||
| 5 | 1 | reservoir | sodium acetate | 0.1 (M) | pH4.6 |
