1NP1
CRYSTAL STRUCTURE OF THE COMPLEX OF NITROPHORIN 1 FROM RHODNIUS PROLIXUS WITH HISTAMINE
Experimental procedure
Source type | ROTATING ANODE |
Source details | ENRAF-NONIUS FR571 |
Temperature [K] | 293 |
Detector technology | DIFFRACTOMETER |
Collection date | 1996-04-10 |
Detector | ENRAF-NONIUS FAST |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 39.550, 74.570, 66.560 |
Unit cell angles | 90.00, 99.94, 90.00 |
Refinement procedure
Resolution | 15.000 - 2.000 |
R-factor | 0.181 |
Rwork | 0.181 |
R-free | 0.27800 |
Structure solution method | MIR |
RMSD bond length | 0.016 |
RMSD bond angle | 30.000 * |
Data reduction software | AGROVATA/ROTAVATA |
Data scaling software | Agrovata |
Phasing software | X-PLOR (3.851) |
Refinement software | X-PLOR (3.851) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 28.000 | 2.140 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.041 * | 0.153 * |
Total number of observations | 71252 * | |
Number of reflections | 25247 | |
<I/σ(I)> | 13.4 | 4.1 |
Completeness [%] | 97.7 | 94 |
Redundancy | 2.8 | 2.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7.5 | 2.8 M AMMONIUM PHOSPHATE, 0.1 M TRIS.HCL, PH 7.5, ROOM TEMPERATURE |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 20 (mg/ml) | |
2 | 1 | drop | potassium phosphate | 10 (mM) | |
3 | 1 | reservoir | ammonium phosphate | 2.6 (M) | |
4 | 1 | reservoir | potassium cacodylate | 0.1 (M) |