1NOS
MURINE INDUCIBLE NITRIC OXIDE SYNTHASE OXYGENASE DOMAIN (DELTA 114), IMIDAZOLE COMPLEX
Experimental procedure
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-1 |
Synchrotron site | SSRL |
Beamline | BL9-1 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1997-06 |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 63.000, 73.800, 92.800 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.100 |
R-factor | 0.205 |
Rwork | 0.205 |
R-free | 0.26100 |
Structure solution method | MULTIPLE ISOMORPHOUS REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 24.300 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | X-PLOR (3.8) |
Refinement software | X-PLOR (3.8) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.200 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.046 * | 0.318 * |
Total number of observations | 112723 * | |
Number of reflections | 25837 | |
<I/σ(I)> | 28 | 4.8 |
Completeness [%] | 99.7 | 100 |
Redundancy | 4.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 6.5 | 32 * | pH 6.5 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10 (mg/ml) | |
10 | 1 | reservoir | PEG MW 4000 | 16 (%) | |
2 | 1 | drop | HEPES | 20 (mM) | |
3 | 1 | drop | IM/M | 50 (mM) | |
4 | 1 | drop | 2.5 (%sat) | ||
5 | 1 | drop | glycerol | 5 (%) | |
6 | 1 | drop | dithiothreitol | 0.5 (mM) | |
7 | 1 | drop | PEG MW 4000 | 8 (%) | |
8 | 1 | reservoir | IM/M | 100 (mM) | |
9 | 1 | reservoir | 5 (%sat) |