1NOO
CYTOCHROME P450-CAM COMPLEXED WITH 5-EXO-HYDROXYCAMPHOR
Experimental procedure
| Temperature [K] | 295 |
| Detector technology | AREA DETECTOR |
| Collection date | 1994-10-25 |
| Detector | SIEMENS-NICOLET X100 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 109.000, 104.300, 36.400 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.200 |
| R-factor | 0.171 |
| Rwork | 0.171 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 22.960 * |
| Data reduction software | XENGEN |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.200 |
| Rmerge | 0.098 |
| Number of reflections | 20570 |
| Completeness [%] | 94.0 |
| Redundancy | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | free interface diffusion method * | 7 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | dithiothreitol | 50 (mM) |
