1NOM
DNA POLYMERASE BETA (POL B) (E.C.2.7.7.7), 31-KD DOMAIN; SOAKED IN THE PRESENCE OF MNCL2 (5 MILLIMOLAR)
Experimental procedure
Source type | ROTATING ANODE |
Source details | RIGAKU RUH2R |
Temperature [K] | 298 |
Detector technology | AREA DETECTOR |
Collection date | 1994-06-17 |
Detector | XUONG-HAMLIN MULTIWIRE |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 120.178, 62.884, 38.286 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 3.000 |
R-factor | 0.184 * |
Structure solution method | DIFFERENCE FOURIER |
Starting model (for MR) | 1bpb |
RMSD bond length | 0.016 |
RMSD bond angle | 22.600 * |
Data reduction software | SDMS |
Data scaling software | SDMS |
Phasing software | TNT (V. 5-D) |
Refinement software | TNT (5-D) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 3.100 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.064 * | 0.166 * |
Number of reflections | 6027 | |
<I/σ(I)> | 8.2 | 1.9 |
Completeness [%] | 97.0 | 92 |
Redundancy | 2.7 | 1.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, sitting drop * | 6.5 | THIS ENTRY DESCRIBES THE STRUCTURE THAT RESULTED WHEN A CRYSTAL OF THE C-TERMINAL 31-KD DOMAIN OF RAT DNA POL BETA (SEE ENTRY 1BPB AND REFERENCE 9) HAD BEEN SOAKED IN THE FOLLOWING SOLUTION FOR 1 MONTH: PEG 1500, 15% HEPES, 75 MILLIMOLAR, PH 7.5 NACL, 50 MILLIMOLAR DTT, 0.1 MILLIMOLAR MNCL2, 5 MILLIMOLAR SEE REFERENCE 3 FOR DETAILS CONCERNING EXPERIMENTAL PROCEDURES, RESULTS, AND DISCUSSION FOR THIS STRUCTURE., pH 6.5 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | PEG3350 | 15-17 (%) | |
2 | 1 | reservoir | citrate | 0.1 (M) | |
3 | 1 | reservoir | 50 (mM) | or 50mM Li2SO4 |