1NOI
COMPLEX OF GLYCOGEN PHOSPHORYLASE WITH A TRANSITION STATE ANALOGUE NOJIRIMYCIN TETRAZOLE AND PHOSPHATE IN THE T AND R STATES
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.5 |
| Synchrotron site | SRS |
| Beamline | PX9.5 |
| Detector technology | IMAGE PLATE |
| Collection date | 1993 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 119.000, 190.000, 88.200 |
| Unit cell angles | 90.00, 109.35, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.500 |
| R-factor | 0.171 |
| Rwork | 0.171 |
| R-free | 0.27300 |
| RMSD bond length | 0.018 |
| RMSD bond angle | 3.400 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 9999.000 * | |
| High resolution limit [Å] | 2.500 * | 2.500 * |
| Rmerge | 0.070 | |
| Total number of observations | 263189 * | |
| Number of reflections | 107826 | |
| Completeness [%] | 88.0 | 74 * |
| Redundancy | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 6.7 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | GPb | 20-40 (mg/ml) | |
| 2 | 1 | 1 | IMP | 1 (mM) | |
| 3 | 1 | 1 | spermine | 1 (mM) | |
| 4 | 1 | 1 | BES | 10 (mM) | |
| 5 | 1 | 1 | EDTA | 0.1 (mM) | |
| 6 | 1 | 1 | sodium azide | 0.02 (%) |
