1NOG

Crystal Structure of Conserved Protein 0546 from Thermoplasma Acidophilum

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2001-02-14
Detector	CUSTOM-MADE
Spacegroup name	P 2 3
Unit cell lengths	89.080, 89.080, 89.080
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.690 - 1.550
R-factor	0.197
Rwork	0.197
R-free	0.21500
Structure solution method	MAD
RMSD bond length	0.010
RMSD bond angle	0.900
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.600
High resolution limit [Å]	1.550	1.550
Rmerge	0.094 *	0.470 *
Total number of observations	341835 *
Number of reflections	62540 *
<I/σ(I)>	21.1	4.4
Completeness [%]	99.0 *	99.8 *
Redundancy	10.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6 *	22 *	Christendat, D., (2000) J.Biol.Chem., 275, 24608. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG4000	10 (%)
2	1	reservoir	sodium acetate	100 (mM)