1NOD
MURINE INDUCIBLE NITRIC OXIDE SYNTHASE OXYGENASE DIMER (DELTA 65) WITH TETRAHYDROBIOPTERIN AND SUBSTRATE L-ARGININE
Experimental procedure
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X12C |
Synchrotron site | NSLS |
Beamline | X12C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 1997-09 |
Detector | BRANDEIS |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 213.000, 213.000, 114.200 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.000 - 2.600 |
R-factor | 0.229 |
Rwork | 0.229 |
R-free | 0.28800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1noc |
RMSD bond length | 0.007 |
RMSD bond angle | 24.300 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | X-PLOR (3.8) |
Refinement software | X-PLOR (3.8) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.700 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.057 * | 0.344 * |
Number of reflections | 45303 | |
<I/σ(I)> | 22 | 3 |
Completeness [%] | 95.3 | 74.6 |
Redundancy | 4.6 | 2.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 7.6 * | 4 * | pH 6.5 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 17 (mg/ml) | |
2 | 1 | drop | Hepps | 40 (mM) | |
3 | 1 | drop | glycerol | 10 (%) | |
4 | 1 | drop | dithiothreitol | 1 (mM) | |
5 | 1 | drop | H4B | 8-10 (mM) | |
6 | 1 | reservoir | MES | 50 (mM) | |
7 | 1 | reservoir | beta-octyl glucoside | 5 (%(w/v)) | |
8 | 1 | reservoir | 21 (%) | or ammonium sulfate |