1NO7
Structure of the Large Protease Resistant Upper Domain of VP5, the Major Capsid Protein of Herpes Simplex Virus-1
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-12-21 |
| Detector | CUSTOM-MADE |
| Wavelength(s) | .97885 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 99.072, 99.072, 454.684 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 * - 2.900 |
| R-factor | 0.255 |
| Rwork | 0.255 |
| R-free | 0.28900 |
| Structure solution method | MAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 23.000 * |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.101 * | 0.205 * |
| Total number of observations | 308549 * | |
| Number of reflections | 47194 | |
| <I/σ(I)> | 7 | 3.3 |
| Completeness [%] | 91.2 | 80.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 8 * | 4 * | sodium acetate, imidazole, pH 6.2, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
| 1 | Vapor diffusion * | 8 * | 4 * | sodium acetate, imidazole, pH 6.2, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | 250 (mM) | ||
| 2 | 1 | drop | Tris | 10 (mM) | pH8.0 |
| 3 | 1 | drop | dithiothreitol | 10 (mM) | |
| 4 | 1 | drop | protein | 20 (mg/ml) | |
| 5 | 1 | reservoir | sodium acetate | 0.8-1 (M) | |
| 6 | 1 | reservoir | imidizole | 100 (mM) | pH6.0-6.8 |
