1NO5
Structure of HI0073 from Haemophilus influenzae, the nucleotide binding domain of the HI0073/HI0074 two protein nucleotidyl transferase.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-BM |
| Synchrotron site | APS |
| Beamline | 17-BM |
| Temperature [K] | 173 |
| Detector technology | CCD |
| Collection date | 2002-04-13 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9800 |
| Spacegroup name | P 63 |
| Unit cell lengths | 89.560, 89.560, 61.480 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.800 * - 1.800 |
| R-factor | 0.201 * |
| Rwork | 0.200 |
| R-free | 0.28200 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 0.034 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.800 * | 1.830 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.028 | 0.269 |
| Total number of observations | 960910 * | |
| Number of reflections | 51005 | |
| <I/σ(I)> | 32.14 | 5 |
| Completeness [%] | 0.0 * | 100 |
| Redundancy | 6.5 | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | 294 | 10% 2-Propanol, 0.1 M Sodium cacodylate, 0.2 M Zn(acetate)2, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | Tris-HCl | 20 (mM) | pH7.5 |
| 2 | 1 | drop | EDTA | 1 (mM) | |
| 3 | 1 | drop | protein | 15 (mg/ml) | |
| 4 | 1 | reservoir | 2-propanol | 11 (%) | |
| 5 | 1 | reservoir | sodium cacodylate | 0.1 (M) | pH6.5 |
| 6 | 1 | reservoir | zinc acetate | 0.2 (M) |
