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1NO4

Crystal Structure of the pre-assembly scaffolding protein gp7 from the double-stranded DNA bacteriophage phi29

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsAPS BEAMLINE 14-BM-C
Synchrotron siteAPS
Beamline14-BM-C
Temperature [K]100
Detector technologyCCD
Collection date2001-11-17
DetectorADSC QUANTUM 4
Wavelength(s)1.000
Spacegroup nameP 21 21 2
Unit cell lengths114.240, 114.164, 60.297
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution60.300 - 2.200
R-factor0.239
Rwork0.239
R-free0.26200
Structure solution methodMAD
RMSD bond length0.006
RMSD bond angle16.400

*

Data reduction softwareDENZO
Data scaling softwareSCALEPACK
Phasing softwareSOLVE
Refinement softwareCNS (1.1)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]60.3002.260
High resolution limit [Å]2.2002.200
Rmerge0.068

*

0.251

*

Number of reflections40285

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<I/σ(I)>19.55
Completeness [%]95.775.4
Redundancy20.06
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1unknown

*

6.52984 % PEG 8000, 0.16 M calcium acetate, 0.08 M sodium cacodylate, 20 % glycerol, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K
Crystallization Reagents in Literatures
IDcrystal IDsolutionreagent nameconcentration (unit)details
111PEG800014.4 (%(v/v))
211sodium cacodylate0.08 (M)pH6.5
311cadmium acetate0.16 (M)
411glycerol20 (%(v/v))
511protein1 (mg/ml)

227561

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