1NNT

STRUCTURAL EVIDENCE FOR A PH-SENSITIVE DI-LYSINE TRIGGER IN THE HEN OVOTRANSFERRIN N-LOBE: IMPLICATIONS FOR TRANSFERRIN IRON RELEASE

Experimental procedure

Spacegroup name	P 21 21 21
Unit cell lengths	46.940, 91.790, 75.820
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 * - 2.300
R-factor	0.16
Rwork	0.160
RMSD bond length	0.015
RMSD bond angle	2.250
Phasing software	X-PLOR
Refinement software	TNT

Data quality characteristics

	Overall
High resolution limit [Å]	2.300 *
Rmerge	0.090 *
Total number of observations	59108 *
Number of reflections	14266 *
Completeness [%]	94.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	5.9 *		used to seed *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	59 (mg/ml)
2	1	drop	acetate	0.02 (M)
3	1	drop		0.01 (%)
4	1	drop	PEG6000	4-5 (%)
5	1	drop	acetate	0.02 (M)
6	1	reservoir	PEG6000	4-5 (%)
7	1	reservoir	acetate	0.02 (M)