1NNS
L-asparaginase of E. coli in C2 space group and 1.95 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LNLS BEAMLINE D03B-MX1 |
| Synchrotron site | LNLS |
| Beamline | D03B-MX1 |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.38 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 76.296, 134.617, 64.867 |
| Unit cell angles | 90.00, 110.51, 90.00 |
Refinement procedure
| Resolution | 13.000 * - 1.950 |
| R-factor | 0.13176 |
| Rwork | 0.130 |
| R-free | 0.17400 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3eca |
| RMSD bond length | 0.014 * |
| RMSD bond angle | 0.032 * |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.09) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 13.000 | 2.000 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.047 | 0.325 |
| Total number of observations | 63394 * | |
| Number of reflections | 40782 | |
| <I/σ(I)> | 2.4 | |
| Completeness [%] | 91.8 | 93.2 * |
| Redundancy | 1.55 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 291 | PEG 3350, MES, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20 (mg/ml) | |
| 2 | 1 | reservoir | MPD | 30 (%) | |
| 3 | 1 | reservoir | PEG3350 | 4 (%) | |
| 4 | 1 | reservoir | MES | 0.1 (M) | pH6 |
