1NNL
Crystal structure of Human Phosphoserine Phosphatase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | BW7B |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2000-09-25 |
Detector | MARRESEARCH |
Wavelength(s) | 0.8423 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 49.033, 130.249, 157.293 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.860 - 1.530 |
R-factor | 0.217 |
Rwork | 0.217 |
R-free | 0.23400 |
Structure solution method | MAD |
RMSD bond length | 0.005 |
RMSD bond angle | 1.144 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHARP |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.560 |
High resolution limit [Å] | 1.530 | 1.530 |
Rmerge | 0.035 | 0.180 |
Number of reflections | 75202 | |
<I/σ(I)> | 27.8 | 3.9 |
Completeness [%] | 99.8 * | 98.7 |
Redundancy | 4.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.3 | 293 | PEG 1500, calcium chloride, cacodylate, pH 6.3, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | 0.7 (M) | ||
2 | 1 | reservoir | cacodylate | 0.1 (M) | pH6.3 |
3 | 1 | reservoir | PEG1500 | 20 (%) | |
4 | 1 | drop | protein | 6.0 (mg/ml) |