1NNL
Crystal structure of Human Phosphoserine Phosphatase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7B |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-09-25 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.8423 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 49.033, 130.249, 157.293 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.860 - 1.530 |
| R-factor | 0.217 |
| Rwork | 0.217 |
| R-free | 0.23400 |
| Structure solution method | MAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.144 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.560 |
| High resolution limit [Å] | 1.530 | 1.530 |
| Rmerge | 0.035 | 0.180 |
| Number of reflections | 75202 | |
| <I/σ(I)> | 27.8 | 3.9 |
| Completeness [%] | 99.8 * | 98.7 |
| Redundancy | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.3 | 293 | PEG 1500, calcium chloride, cacodylate, pH 6.3, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | 0.7 (M) | ||
| 2 | 1 | reservoir | cacodylate | 0.1 (M) | pH6.3 |
| 3 | 1 | reservoir | PEG1500 | 20 (%) | |
| 4 | 1 | drop | protein | 6.0 (mg/ml) |
