1NNI
Azobenzene Reductase from Bacillus subtilis
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 150 |
Detector technology | CCD |
Collection date | 2002-08-31 |
Detector | SBC-2 |
Wavelength(s) | 0.97940, 0.97926, 0.95372 |
Spacegroup name | P 62 2 2 |
Unit cell lengths | 112.720, 112.720, 90.011 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 48.810 - 2.500 |
R-factor | 0.223 |
Rwork | 0.221 |
R-free | 0.23800 |
Structure solution method | MAD |
RMSD bond length | 0.009 |
RMSD bond angle | 1.600 |
Data reduction software | d*TREK |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.500 |
Number of reflections | 19743 |
Completeness [%] | 89.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | trisodium citrate, sodium HEPES, sucrose, pH 7.5-8.5, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |