1NNH
Hypothetical protein from Pyrococcus furiosus Pfu-1801964
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-10-19 |
| Detector | MARRESEARCH |
| Spacegroup name | I 41 |
| Unit cell lengths | 68.529, 68.529, 150.684 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 62.020 - 1.650 |
| R-factor | 0.172 |
| Rwork | 0.171 |
| R-free | 0.19100 |
| Structure solution method | SAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.390 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.710 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Number of reflections | 41526 | |
| <I/σ(I)> | 46.8 | 7.2 |
| Completeness [%] | 100.0 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 9 | 291 | PEG 550 MME, sodium chloride, bicine, pH 9, modified microbatch, temperature 291K, pH 9.00 |
