1NNF
Crystal Structure Analysis of Haemophlius Influenzae Ferric-ion Binding Protein H9Q Mutant Form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.3 |
Synchrotron site | ALS |
Beamline | 5.0.3 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-06-29 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 105.101, 75.344, 33.503 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 105.410 - 1.100 |
R-factor | 0.15934 |
Rwork | 0.158 |
R-free | 0.18040 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1d9v |
RMSD bond length | 0.010 |
RMSD bond angle | 1.257 |
Data reduction software | TRUNCATE |
Data scaling software | CCP4 ((TRUNCATE)) |
Phasing software | MOLREP |
Refinement software | REFMAC (5.1.19) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 105.410 | 1.140 |
High resolution limit [Å] | 1.100 | 1.100 |
Rmerge | 0.044 * | 0.199 * |
Number of reflections | 89423 | |
Completeness [%] | 86.2 * | 36.1 |
Redundancy | 6.7 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.2 | 20 * | PEG 5000, Tris HCl, pH 8.2, VAPOR DIFFUSION, SITTING DROP, temperature 293.0K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | PEG5000 | 10 (%(w/v)) | |
2 | 1 | drop | Tris-HCl | 50 (mM) | pH8.2 |
3 | 1 | reservoir | PEG5000 | 20 (%) | |
4 | 1 | reservoir | Tris-HCl | 100 (mM) | pH8.2 |