1NND
Arginine 116 is Essential for Nucleic Acid Recognition by the Fingers Domain of Moloney Murine Leukemia Virus Reverse Transcriptase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-01-15 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 38.795, 116.652, 61.652 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.840 - 2.300 |
| R-factor | 0.21 |
| Rwork | 0.203 |
| R-free | 0.27400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1QAI MOLECULE A |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.253 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.050 * | 0.130 * |
| Total number of observations | 58875 * | |
| Number of reflections | 12471 * | |
| <I/σ(I)> | 13.6 | |
| Completeness [%] | 95.8 * | 83.9 |
| Redundancy | 9.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7 | 20 * | PEG 400, Magnesium Chloride, HEPES, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 0.55 (mM) | |
| 2 | 1 | drop | 5'-d(CATGCATG)-3' | 1.1 (mM) | |
| 3 | 1 | drop | PEG400 | 12-18 (%) | |
| 4 | 1 | drop | HEPES | 50 (mM) | pH7.0 |
| 5 | 1 | drop | 5 (mM) | ||
| 6 | 1 | reservoir | PEG400 | 2-6 (%) |
