1NNC

INFLUENZA VIRUS NEURAMINIDASE SUBTYPE N9 (TERN) COMPLEXED WITH 4-GUANIDINO-NEU5AC2EN INHIBITOR

Experimental procedure

Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-6A
Synchrotron site	Photon Factory
Beamline	BL-6A
Wavelength(s)	1.0
Spacegroup name	I 4 3 2
Unit cell lengths	182.800, 182.800, 182.800
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 - 1.800
R-factor	0.156
Rwork	0.156
RMSD bond length	0.010
RMSD bond angle	1.660
Data reduction software	WEIS
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
High resolution limit [Å]	1.800 *	1.600 *
Rmerge	0.090 *	0.170 *
Total number of observations	106232 *
Number of reflections	29729
Completeness [%]	63.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	6.6 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10-15 (mg/ml)
2	1	drop	potassium phosphate	1.7 (M)
3	1	reservoir	potassium phosphate	1.9 (M)