1NMP
Structural genomics, ybgI protein, unknown function
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-07-02 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 3 |
| Unit cell lengths | 156.730, 156.730, 57.610 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 * - 2.200 |
| R-factor | 0.212 |
| Rwork | 0.212 |
| R-free | 0.25200 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | SeMet structure |
| RMSD bond length | 0.026 |
| RMSD bond angle | 24.200 * |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | |
| High resolution limit [Å] | 2.200 | |
| Rmerge | 0.096 | 0.237 * |
| Total number of observations | 303926 * | |
| Number of reflections | 80094 | |
| <I/σ(I)> | 14.1 | |
| Completeness [%] | 99.8 * | 99.6 * |
| Redundancy | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 0.1M cacodylate buffer pH 7.5, 0.1M magnesium acetate, 15% (w/v) polyethylene glycol 8000, 5% (v/v) polyethylene glycol 400, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | cacodylate | 0.1 (M) | pH7.5 |
| 2 | 1 | reservoir | magnesium acetate | 0.1 (M) | |
| 3 | 1 | reservoir | PEG8000 | 15 (%(w/v)) | |
| 4 | 1 | reservoir | PEG400 | 5 (%(v/v)) | |
| 5 | 1 | drop | protein | 4.7 (mg/ml) |
