1NMO

Structural genomics, protein ybgI, unknown function

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2002-07-01
Detector	MARRESEARCH
Wavelength(s)	0.9895,0.9793,0.9780
Spacegroup name	P 3
Unit cell lengths	154.660, 154.660, 57.520
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 * - 2.200
R-factor	0.211
Rwork	0.211
R-free	0.26000 *
Structure solution method	MAD
RMSD bond length	0.025
RMSD bond angle	2.400 *
Data scaling software	d*TREK
Phasing software	RESOLVE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000
High resolution limit [Å]	2.200
Rmerge	0.060	0.216 *
Total number of observations	298987 *
Number of reflections	77981 *
<I/σ(I)>	25.6
Completeness [%]	97.6 *	86.3 *
Redundancy	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	298	0.1M imidazole, 0.2M calcium acetate, 15% (w/v) polyethylene glycol 3350, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	imidazole	0.1 (M)	pH8.0
2	1	reservoir	calcium acetate	0.2 (M)
3	1	reservoir	PEG3350	15 (%(w/v))
4	1	drop	protein	8.2 (mg/ml)