1NMO
Structural genomics, protein ybgI, unknown function
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-07-01 |
Detector | MARRESEARCH |
Wavelength(s) | 0.9895,0.9793,0.9780 |
Spacegroup name | P 3 |
Unit cell lengths | 154.660, 154.660, 57.520 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.000 * - 2.200 |
R-factor | 0.211 |
Rwork | 0.211 |
R-free | 0.26000 * |
Structure solution method | MAD |
RMSD bond length | 0.025 |
RMSD bond angle | 2.400 * |
Data scaling software | d*TREK |
Phasing software | RESOLVE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | |
High resolution limit [Å] | 2.200 | |
Rmerge | 0.060 | 0.216 * |
Total number of observations | 298987 * | |
Number of reflections | 77981 * | |
<I/σ(I)> | 25.6 | |
Completeness [%] | 97.6 * | 86.3 * |
Redundancy | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | 0.1M imidazole, 0.2M calcium acetate, 15% (w/v) polyethylene glycol 3350, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | imidazole | 0.1 (M) | pH8.0 |
2 | 1 | reservoir | calcium acetate | 0.2 (M) | |
3 | 1 | reservoir | PEG3350 | 15 (%(w/v)) | |
4 | 1 | drop | protein | 8.2 (mg/ml) |