1NMD
Crystal Structure of D. Discoideum Actin-Gelsolin Segment 1 Complex Crystallized In Presence Of Lithium ATP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X9B |
| Synchrotron site | NSLS |
| Beamline | X9B |
| Temperature [K] | 93 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-06-17 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.97946 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 178.912, 69.053, 56.613 |
| Unit cell angles | 90.00, 104.65, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.900 |
| Rwork | 0.203 |
| R-free | 0.25040 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1DGA |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.403 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.930 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.072 | 0.365 |
| Number of reflections | 52529 | |
| Completeness [%] | 99.7 | 96.1 |
| Redundancy | 2.61 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 300 | Lithium sulfate, HEPES, ATP, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 300K |
