1NM1
Crystal Structure of D. Dicsoideum Actin Complexed With Gelsolin Segment 1 and Mg ATP at 1.8 A Resolution
Replaces: 1DGAExperimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X9B |
| Synchrotron site | NSLS |
| Beamline | X9B |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 1999-04-19 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.07018 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 178.539, 69.089, 56.552 |
| Unit cell angles | 90.00, 104.34, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.800 |
| Rwork | 0.197 |
| R-free | 0.23180 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1DGA |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.412 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.830 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.044 | 0.207 |
| Number of reflections | 60943 | |
| Completeness [%] | 98.2 | 81.8 |
| Redundancy | 2.41 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | Magnesium chloride, litheim chloride, HEPES, ATP, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
