1NLZ

Crystal structure of unliganded traffic ATPase of the type IV secretion system of helicobacter pylori

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-BM
Synchrotron site	APS
Beamline	19-BM
Temperature [K]	100
Detector technology	CCD
Detector	CUSTOM-MADE
Wavelength(s)	0.9793,0.9794,0.9667
Spacegroup name	P 31
Unit cell lengths	104.530, 104.530, 168.720
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 * - 3.000
R-factor	0.239
Rwork	0.239
R-free	0.29800
Structure solution method	MAD
RMSD bond length	0.013 *
RMSD bond angle	1.530 *
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	3.100
High resolution limit [Å]	3.000	3.000
Rmerge	0.083 *	0.382 *
Total number of observations	291962 *
Number of reflections	74172
Completeness [%]	89.4 *	88.7 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	6.9	298	PEG MME 2000, NaCl, Tris-HCl,glycerol, pH 6.9, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG2000 MME	18 (%)
2	1	reservoir		0.1 (M)
3	1	reservoir	Tris-HCl	200 (mM)	pH6.9