1NLW
Crystal structure of Mad-Max recognizing DNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Detector technology | CCD |
Wavelength(s) | 0.92 |
Spacegroup name | P 1 |
Unit cell lengths | 47.346, 56.020, 65.602 |
Unit cell angles | 88.82, 79.08, 67.11 |
Refinement procedure
Resolution | 20.000 - 2.000 |
R-factor | 0.264 |
Rwork | 0.264 |
R-free | 0.32400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1nkp |
RMSD bond length | 0.006 |
RMSD bond angle | 0.940 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | |
High resolution limit [Å] | 2.000 | |
Rmerge | 0.052 | |
Total number of observations | 214612 * | |
Number of reflections | 35483 | |
Completeness [%] | 88.8 | 59.9 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 15 * | 20% MPD, 5mM magnesium chloride, 50 mM sodium cacodylate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 288K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | 20% methyl pentane diol | ||
2 | 1 | 1 | 5MM magnesium chloride | ||
3 | 1 | 1 | 50MM sodium cacodylate pH 6.0 | ||
4 | 1 | 2 | methyl pentane diol | ||
5 | 1 | 2 | magnesium chloride | ||
6 | 1 | 2 | sodium cacodylate pH 6.0 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | MPD | 20 (%) | |
2 | 1 | reservoir | 5 (mM) | ||
3 | 1 | reservoir | sodium cacodylate | 50 (mM) | pH6.0 |
4 | 1 | drop | protein | 10 (mg/ml) |